anti Phosphoserine/threonine 磷酸化丝氨酸/苏氨酸抗体 PP2551 ECM Biosciences

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Anti-Phosphoserine/threonine Rabbit Polyclonal Antibody

品牌/厂商:ECM Biosciences

货号/目录号:PP2551

规格单位:100 uL

抗体来源:Rabbit 兔多克隆抗体

种属反应性:Hu, Rt, Ms

ECM Biosciences产品链接

资料背景简介

Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.

特定丝氨酸或苏氨酸残基的磷酸化是重要的翻译后修饰,用于调节大多数蛋白质的活性。各种细胞信号通路的刺激激活介导这些蛋白质修饰的受体和非受体ser / thr激酶。可以检测磷酸丝氨酸或磷酸苏氨酸残基的抗体是表征多种磷酸化蛋白翻译后状态变化的极佳工具。通常使用感兴趣的蛋白质进行免疫沉淀,然后使用抗磷酸丝氨酸抗体检测磷酸丝氨酸或磷酸苏氨酸,以将磷酸化状态的变化与蛋白质活性的变化相关联。

参考文献

Hunter T.(1987) Cell. 50(6):823.

Krishna, R.G. & Wold, F. (1993) Adv Enzymol Rel Areas Mol Biol 67:265.

Yaffe, M.B. & Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.

抗原

Anti-Phosphoserine/threonine was generated from a panel of phosphoserine and phosphothreonine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved in many species.

缓冲液和保存成分

Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

免疫性和应用

This antibody was cross-adsorbed to unphosphorylated peptide then affinity purified using a mix of phosphoserine and phosphothreonine peptides (without carrier). The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA.

咨询和订购:

北京华泰昕生物医疗技术有限公司,Tel: 010-83131025, email: info@hypercyte.com, QQ1506992028

不同应用稀释比例:

ELISA1:2000
ICC1:50
IP1:100
WB1:1000

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