Asc(Tyr-144), 磷酸化特异性抗体, AP5631


Host- and pathogen-associated cytoplasmic double-stranded DNA triggers the activation of a NALP3-independent inflammasome, which activates caspase-1, leading to maturation of pro-interleukin-1beta and inflammation. Several studies have isolated AIM2 (absent in melanoma 2) as a candidate cytoplasmic-DNA-sensing protein that contains an N-terminal pyrin domain and C-terminal oligonucleotide binding domain. A screen for transcripts induced by interferon-beta identified AIM2 gene expression. AIM2 protein bound double-stranded DNA, recruited the inflammasome adaptor ASC, and localized to ASC containing speckles. AIM2 and ASC form a pyroptosome, which induces pyroptotic cell death mediated by caspase-1. Asc can be phosphorylated at Tyr-144 in a Syk and JNK-dependent manner. This phosphorylation is critical for Asc speck formation and Caspase-1 activation.


Hara, H. et al. (2013) Nature Immunol. 14(12):1247. 
Roberts, T.L. et al. (2009). Science. 323(5917):1057.
Bürckstümmer, T. et al. (2009). Nat Immunol. 10(3):266.


Western blot analysis of mouse macrophage J774A.1 cells stimulated with pervanadate (1 mM for 30 min.), then untreated (-) or treated (+) with alkaline phosphatase. The blot was probed with rabbit polyclonal anti-Asc (Tyr-144) phospho-specific antibody (AP5631) at 1:500.
Immunocytochemical labeling of Asc (Tyr-144) in inflammasomes. Paraformaldehyde fixed J774 cells were primed with LPS and treated with nigericin. Cells were co-labeled with DAPI, a caspase-1 inhibitor (FAM-YVAD-FMK), and anti-Asc (Tyr-144) phosphospecific antibody detected with AlexaFluor 568 secondary. (Image provided by Jordan Yaron, Center for Biosignatures Discovery Automation, Arizona State University)

序列:Asc (Tyr-144) phospho-peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-144 in mouse Asc. This peptide sequence is highly conserved in human and rat Asc. 

*For more information, see UniProt Accession Q9EPB4


Yu, SH et al. (2019) J Ethnopharmacol. 239:111917. (WB: mouse bone marrow macrophages)
Dubois, EC et al. (2019) PLoS Pathog. 15(4):e1007709. (WB: mouse macrophage)
Furuya, MY et al. (2018) Arthritis Res Ther. 20(1):196. (WB: human neutrophils)
Kwak, SB et al. (2018) Mediators Inflamm. 2018:6054069. (WB: mouse J774A.1, BMDMs)
Hoyt, L.R. et al. (2016) J Immunol. 197:1322. (WB: mouse macrophage, LPS)


供应商:北京华泰昕生物医疗技术有限公司(ECM Biosciences)